Crystallization (Comment) | Organism |
---|---|
ATX alone and in complex with LPAs with different acyl-chain lengths and saturations, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F249A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
F274A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
L213A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
L243A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
M512A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
N230A | almost inactive mutant | Mus musculus |
W254A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
Y306A | the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
More | autotaxin domain arrangement and structure, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
ATX | - |
Mus musculus |
autotaxin | - |
Mus musculus |
ENPP2 | - |
Mus musculus |
lysoPLD | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
additional information | a loop region in the catalytic domain is a major determinant for the substrate specificity of the Enpp family enzymes, Asn230 is critical for the recognition of the common phosphate moieties, structure of the ATX active site in the free-form and with bound Zn2+, overview | Mus musculus |
physiological function | autotaxin is a secreted lysophospholipase D that hydrolyzes lysophosphatidylcholine to generate lysophosphatidic acid, a lipid mediator that activates G protein-coupled receptors to evoke various cellular responses. The produced LPAs are delivered from the active site to cognate G protein-coupled receptors through a hydrophobic channel | Mus musculus |